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anti vac14 (Proteintech)

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Proteintech anti vac14
Anti Vac14, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vac14/VAC14+Antibody/pm41592073-235-126-129
Average 94 stars, based on 5 article reviews

anti vac14 - by Bioz Stars, 2026-09

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Article Title: TIG1 Inhibits the mTOR Signaling Pathway in Malignant Melanoma Through the VAC14 Protein.
Article Snippet: Background/Aim: Currently, there are few drug options available to treat malignant melanoma.. Tazaroteneinducible gene 1 (TIG1) was originally isolated from skin tissue, but its function in skin tissue has not been clarified.. The aim of this study was to elucidate the effect of TIG1 and mTOR signaling pathways associated with VAC14 on melanoma.

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Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model.
Article Snippet: .. The following antibodies were used for western blot analyses: anti-Flag (M20008, 1:2000, Abmart, RRID: AB_2713960), anti-HA (ab9110, 1:2000, Abcam, RRID: AB_307019), anti-Strep II (HA500061, 1:2000, HUABIO, RRID: AB_2904531), anti-GAPDH (AC002, 1:10000, ABclonal, RRID: AB_2736879), anti-Sodium Potassium ATPase (ab76020, 1:10000, Abcam, RRID: AB_1310695), anti-HSD17B12 (ab236990, 1:2000, Abcam, RRID: AB_2876866), anti-PD-L1 (13684, 1:2000, CST, RRID: AB_2687655), anti-PD-L1 (ab213480, 1:2000, Abcam, RRID: AB_2773715), anti-MHC-I (ET170247, 1:1000, HUABIO, RRID: AB_3070309), anti-ALIX (67715-1-Ig, 1:2000, Proteintech, RRID: AB_2882905), anti-CD9 (60232-1-Ig, 1:2000, Proteintech, RRID: AB_11232215), anti-CD9 (ET1601-9, 1:2000, HUABIO, RRID: AB_3069620), anti-PDI (WH319294, 1:1000, ABclonal), anti-ATG7 (T55658S, 1:2000, Abmart), anti-biotin (GTX124262-S, 1:5000, GeneTex, RRID: AB_11174491), Streptavidin-HRP (A0303, 1:2000, Beyotime), anti-Ub (sc-8017, 1:200, Santa Cruz Biotechnology, RRID: AB_628423), anti-CMTM6 (HPA026980, 1:1000, Sigma, RRID: AB_10602801), anti-HRS (PTG-10390, 1:2000, Proteintech, RRID: AB_2118914), anti-VPS28 (ab167172, 1:2000, Abcam), anti-VAC14 (PTG-15771, 1:1000, Proteintech, RRID: AB_2214753), HRP conjugated goat anti-rabbit IgG (H + L) (SA00001–2, 1:2000, Proteintech, RRID: AB_2722564), HRP conjugated goat anti-mouse IgG (H + L) (31,430, 1:2000, Thermofisher, RRID: AB_228307). .. PLOS Biology | https://doi.org/10.1371/journal.pbio.3003603 January 27, 2026 15 / 25 The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500).

Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model
Article Snippet: .. The following antibodies were used for western blot analyses: anti-Flag (M20008, 1:2000, Abmart, RRID: AB_2713960), anti-HA (ab9110, 1:2000, Abcam, RRID: AB_307019), anti-Strep II ( HA500061 , 1:2000, HUABIO, RRID: AB_2904531), anti-GAPDH (AC002, 1:10000, ABclonal, RRID: AB_2736879), anti-Sodium Potassium ATPase (ab76020, 1:10000, Abcam, RRID: AB_1310695), anti-HSD17B12 (ab236990, 1:2000, Abcam, RRID: AB_2876866), anti-PD-L1 (13684, 1:2000, CST, RRID: AB_2687655), anti-PD-L1 (ab213480, 1:2000, Abcam, RRID: AB_2773715), anti-MHC-I (ET1702-47, 1:1000, HUABIO, RRID: AB_3070309), anti-ALIX (67715-1-Ig, 1:2000, Proteintech, RRID: AB_2882905), anti-CD9 (60232-1-Ig, 1:2000, Proteintech, RRID: AB_11232215), anti-CD9 (ET1601-9, 1:2000, HUABIO, RRID: AB_3069620), anti-PDI (WH319294, 1:1000, ABclonal), anti-ATG7 (T55658S, 1:2000, Abmart), anti-biotin (GTX124262-S, 1:5000, GeneTex, RRID: AB_11174491), Streptavidin-HRP (A0303, 1:2000, Beyotime), anti-Ub (sc-8017, 1:200, Santa Cruz Biotechnology, RRID: AB_628423), anti-CMTM6 (HPA026980, 1:1000, Sigma, RRID: AB_10602801), anti-HRS (PTG-10390, 1:2000, Proteintech, RRID: AB_2118914), anti-VPS28 (ab167172, 1:2000, Abcam), anti-VAC14 (PTG-15771, 1:1000, Proteintech, RRID: AB_2214753), HRP conjugated goat anti-rabbit IgG (H + L) (SA00001–2, 1:2000, Proteintech, RRID: AB_2722564), HRP conjugated goat anti-mouse IgG (H + L) (31,430, 1:2000, Thermofisher, RRID: AB_228307). .. The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500).

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A DMSO-differentiated cells were pretreated with DHBP (an inhibitor of endoplasmic reticulum calcium release), ML-SI1 (a TRPML1 inhibitor) or BAPTA-AM (a calcium chelator) for 6 h before under RFC conditions, thereafter the total calcium ion (Ca²⁺) flux was determined by Flow cytometry analysis. Data are presented as mean ± SD. Statistical significance was determined using ANOVA. * P < 0.05, ** P < 0.01. B The effects of shRNA for TRPML1 on calcium flux in HepaRG cells (DMSO was used to induce differentiation) under RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. C Immunofluorescence staining of AFP (red) and DAPI (blue) in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Scale bar: 80 μm. Mean ± SD by ANOVA. D The RT-qPCR analysis for mRNA levels of AFP in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Mean ± SD by ANOVA. E The western blot analysis for protein content of AFP in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Mean ± SD by ANOVA. F The effects of YM201636 on calcium flux in HepaRG cells under RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. G , H The mRNA and protein levels of VAC14 in HepaRG cells were incubated without or with RFC (10 rpm/min) for 72 h as assessed by RT-qPCR assay and immunofluorescence staining. Scale bar: 80 μm. Mean ± SD by ANOVA. I The RT-qPCR analysis for mRNA level of AFP in HepaRG cells pretreated without or with YM201636 (1 µM) under RFC or not. Mean ± SD by Student’s t -test. J The immunofluorescence staining of AFP in HepaRG cells pretreated without or with YM201636 (1 µM) under RFC conditions or not. Scale bar: 80 μm. Mean ± SD by ANOVA. K The western blot analysis of AFP content in HepaRG cells pretreated without or with YM201636 (1 µM), under RFC or not. The data are based on three independent experiments. * P < 0.05, ** P < 0.01.

Journal: NPJ Microgravity

Article Title: TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions

doi: 10.1038/s41526-025-00461-4

Figure Lengend Snippet: A DMSO-differentiated cells were pretreated with DHBP (an inhibitor of endoplasmic reticulum calcium release), ML-SI1 (a TRPML1 inhibitor) or BAPTA-AM (a calcium chelator) for 6 h before under RFC conditions, thereafter the total calcium ion (Ca²⁺) flux was determined by Flow cytometry analysis. Data are presented as mean ± SD. Statistical significance was determined using ANOVA. * P < 0.05, ** P < 0.01. B The effects of shRNA for TRPML1 on calcium flux in HepaRG cells (DMSO was used to induce differentiation) under RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. C Immunofluorescence staining of AFP (red) and DAPI (blue) in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Scale bar: 80 μm. Mean ± SD by ANOVA. D The RT-qPCR analysis for mRNA levels of AFP in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Mean ± SD by ANOVA. E The western blot analysis for protein content of AFP in HepaRG cells pretreated without or with BAPTA-AM (2.5 µM) under RFC or not. Mean ± SD by ANOVA. F The effects of YM201636 on calcium flux in HepaRG cells under RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. G , H The mRNA and protein levels of VAC14 in HepaRG cells were incubated without or with RFC (10 rpm/min) for 72 h as assessed by RT-qPCR assay and immunofluorescence staining. Scale bar: 80 μm. Mean ± SD by ANOVA. I The RT-qPCR analysis for mRNA level of AFP in HepaRG cells pretreated without or with YM201636 (1 µM) under RFC or not. Mean ± SD by Student’s t -test. J The immunofluorescence staining of AFP in HepaRG cells pretreated without or with YM201636 (1 µM) under RFC conditions or not. Scale bar: 80 μm. Mean ± SD by ANOVA. K The western blot analysis of AFP content in HepaRG cells pretreated without or with YM201636 (1 µM), under RFC or not. The data are based on three independent experiments. * P < 0.05, ** P < 0.01.

Article Snippet: Antibodies used in this study were as follows: AFP (Proteintech, 14550-1-AP), ALB (Proteintech, 16475-1-AP), CK18 (Proteintech, 10830-1-AP), TRPML1 (Santa Cruz Biotechnology, sc-398868), VAC14 (Santa Cruz Biotechnology, sc-271831), F-actin (ThermoFisher, A30107), β-cantein (Proteintech, 51067-2-AP), FAK (ABclonal, A11131), β-actin (Proteintech, 81115-1-RR), FN1 (ABclonal, A12932), FN1 (ABclonal, A12932), VIM (ABclonal, A19607), α-SMA (Proteintech, 14395-1-AP), GAPDH (Proteintech, 60004-1-Ig), and all the Primary antibodies were at 1:1000 dulution.

Techniques: Flow Cytometry, shRNA, Immunofluorescence, Staining, Quantitative RT-PCR, Western Blot, Incubation

A The RT-qPCR analysis for the mRNA levels of FAK, β-actin, FN1, VIM, and α-SAM in HepaRG cells, without or with RFC. Mean ± SD ( n = 3) by Student’s t -test. B The western blot analysis for the protein expression of FAK, β-actin, FN1, VIM, and α-SAM in HepaRG cells, without or with RFC. C Immunofluorescence staining of F-action (green) and DAPI (blue) in HepaRG cells incubated without or with RFC (10 rpm/min) for 72 h as assessed by confocal imaging. Scale bar: 80 μm. Mean ± SD by Student’s t -test. D The AFM analysis for the stiffness of HepaRG cells, without or with RFC. Mean ± SD ( n = 5) by Student’s t -test. E The effects of cytoskeletal remodeling on calcium flux in HepaRG cells treated without or with RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. F The content of AFP in HepaRG cells incubated without or with Cyt B, under RFC conditions or not, as assessed by immunofluorescence staining (Con: the undifferentiated group, the other groups: differentiated with DMSO). Scale bar: 80 μm. Mean ± SD by ANOVA. G The content of VAC14 in HepaRG cells incubated without or with Cyt B, under RFC conditions or not, as assessed by immunofluorescence staining. Scale bar: 20 μm. Mean ± SD by ANOVA. The data are based on three independent experiments. * P < 0.05, ** P < 0.01.

Journal: NPJ Microgravity

Article Title: TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions

doi: 10.1038/s41526-025-00461-4

Figure Lengend Snippet: A The RT-qPCR analysis for the mRNA levels of FAK, β-actin, FN1, VIM, and α-SAM in HepaRG cells, without or with RFC. Mean ± SD ( n = 3) by Student’s t -test. B The western blot analysis for the protein expression of FAK, β-actin, FN1, VIM, and α-SAM in HepaRG cells, without or with RFC. C Immunofluorescence staining of F-action (green) and DAPI (blue) in HepaRG cells incubated without or with RFC (10 rpm/min) for 72 h as assessed by confocal imaging. Scale bar: 80 μm. Mean ± SD by Student’s t -test. D The AFM analysis for the stiffness of HepaRG cells, without or with RFC. Mean ± SD ( n = 5) by Student’s t -test. E The effects of cytoskeletal remodeling on calcium flux in HepaRG cells treated without or with RFC (10 rpm/min) for 72 h. Mean ± SD by Student’s t -test. F The content of AFP in HepaRG cells incubated without or with Cyt B, under RFC conditions or not, as assessed by immunofluorescence staining (Con: the undifferentiated group, the other groups: differentiated with DMSO). Scale bar: 80 μm. Mean ± SD by ANOVA. G The content of VAC14 in HepaRG cells incubated without or with Cyt B, under RFC conditions or not, as assessed by immunofluorescence staining. Scale bar: 20 μm. Mean ± SD by ANOVA. The data are based on three independent experiments. * P < 0.05, ** P < 0.01.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Staining, Incubation, Imaging

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